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Physiological processes

Study ligand – cell interactions in a physiological environment

Investigate how the strength and kinetics of your interaction is affected by temperature. Reduce the temperature to turn off cellular processes such as internalization, or increase it to 37 °C to approach an in vivo setting. Understand your system better with the use of labels, such as pH sensitive dyes or radionuclides that can be excreted.

Gained information

  • Temperature effects on the affinity
  • Temperature effects on the binding kinetics
  • Signs of internalization rate
  • Nuclide excretion rate as the result of internalization, degradation, or cell death.

Publications

Resolving the EGF-EGFR interaction characteristics through a multiple-temperature, multiple-inhibitor, real-time interaction analysis approach.
Björkelund H, Gedda L, Malmqvist M, Andersson K.
Mol Clin Onc. 2013. 1: 343-352
PubMed

Helicobacter pylori adapts to chronic infection and gastric disease via pH-Responsive BabA-mediated adherence.
Bugaytsova JA et al.
Cell Host Microbe. 2017. 21(3):376-389.
PubMed