Protein – protein interactions measured with LigandTracer
Working with purified proteins is suitable when the target protein is not expressed on a cell surface. The cell-free environment also enables a highly controlled setting, ideal for quality control measurements. The target proteins may be attached through adhesion, immobilized on a chip or on magnetic beads.
Transitional changes in the CRP structure lead to the exposure of proinflammatory binding sites.
Braig D, Nero T L, Koch HG, Kaiser B, Wang X, Thiele JR, Morton CJ, Zeller J, Kiefer J, Potempa LA, Mellett NA, Miles LA, Du XJ, Meikle PJ, Huber-Lang M, Stark GB, Parker MW, Peter K, Eisenhardt SU.
Nat Commun. 2017. 8:14188.
Automated functional characterization of radiolabeled antibodies: a time-resolved approach.
Wang E, Björkelund H, Mihaylova D, Hagemann UB, Karlsson J, Malmqvist M, Buijs J, Abrahmsén L, Andersson K.
Nucl Med Commun. 2014. 35(7):767-776.