Specificity and competition
Investigate specificity and competitive behavior of your binder
Confirming the specificity of a detected binding is crucial. Compare positive and negative cell lines within the same measurement or pre-incubate with a non-labeled ligand to confirm self-competition. Investigate if two molecules bind to the same epitope by keeping one non-labeled and follow competition (shared epitope) or label with a quencher* and follow binding in proximity (different epitopes).
*Only in LigandTracer Green
- Cell line specificity – absence of ligand on cells lacking target receptors
- Target specificity – the possibility to self-compete
- Epitope mapping – competition between molecules
- Epitope mapping – quenching of molecules in close proximity
Technical Aspect of the Automated Synthesis and Real-Time Kinetic Evaluation of [11C]SNAP-7941.
Vraka C, Pichler V, Pfaff S, Balber T, Hacker M, Mitterhauser M, Wadsak W, Philippe C.
J. Vis. Exp. 2019. 146
A novel real-time proximity assay for characterizing multiple receptor interactions on living cells.
Bondza S, Björkelund H, Nestor M, Andersson K, Buijs J.
Anal Chem. 2017. 89(24):13212-13218