Affinity and kinetics
Study ligand-receptor interactions in real-time to learn about the affinity and kinetics
Knowledge about the affinity and kinetics of interactions is of great value when ranking drug leads, developing radiotracers or investigating a receptor system. Fit curves with the model that best describes the biology – most likely found in our large library of models.
Gained information
- The affinity of the interaction (KD)
- Kinetic rate constants (ka and kd)
- The ligand residence time (t1/2)
- Possible deviations from a one-to-one binding mode
- The concentration required to saturate targets
Key publications
These publications illustrate the application well. Click here for more examples.
Real-time characterization of antibody binding to receptors on living immune cells.
Bondza S, Foy E, Brooks J, Andersson K, Robinson J, Richalet P, Buijs J.
Front. Immunol. 2017. 8(455).
Summary
PubMed
Development of human monoclonal antibody for Claudin-3 overexpressing carcinoma targeting.
Yang H, Park H,Jin Lee Y, Young Choi J, Kim TE, Rajasekaran N, Lee S, Song K, Hong S, Choi J-S, Shim H, Kim Y-D, Hwang S, Choi Y-L, Kee Shin Y.
Biomolecules. 2020. 10(1): 51.
Summary
PubMed
Brain delivery of biologics using a cross‐species reactive transferrin receptor 1 vnar shuttle.
Sehlin D, Stocki P, Gustavsson T, Hultqvist G, Walsh FS, Rutkowski JL, Syvänen S.
FASEB J. 2020. 34(10):13272-13283.
Summary
PubMed
Examples of insights
