icon flag insights

Affinity and kinetics

Study ligand-receptor interactions in real-time to learn about the affinity and kinetics

Knowledge about the affinity and kinetics of interactions is of great value when ranking drug leads, developing radiotracers or investigating a receptor system. Fit curves with the model that best describes the biology – most likely found in our large library of models.

Click images to enlarge
Accurate estimations of the binding kinetics and affinity are typically obtained from curves with two incubation phases and one wash-out phase. Here FITC-Rituximab binding to anchored B-cells (n=4).

Gained information

  • The affinity of the interaction (KD)
  • Kinetic rate constants (ka and kd)
  • The ligand residence time (t1/2)
  • Possible deviations from a one-to-one binding mode
  • The concentration required to saturate targets



Real-time characterization of antibody binding to receptors on living immune cells.
Bondza S, Foy E, Brooks J, Andersson K, Robinson J, Richalet P, Buijs J.
Front. Immunol. 2017. 8(455).

Development of Human Monoclonal Antibody for Claudin-3 Overexpressing Carcinoma Targeting.
Yang H, Park H,Jin Lee Y, Young Choi J, Kim TE, Rajasekaran N, Lee S, Song K, Hong S, Choi J-S, Shim H, Kim Y-D, Hwang S, Choi Y-L, Kee Shin Y.
Biomolecules. 2020. 10(1): 51.