Screening of focused ligand libraries
Identify potential drug candidates that interact with the selected target in a quantitative kinetics binding assay
Ligand screening is an essential step in drug discovery, where robust methodology and high-quality data are essential for validating molecular interactions in a live cell environment. With LigandTracer, the interaction affinity and kinetics of focused ligand libraries can be quantified directly on cells, tissue, or immobilized targets in a real-time binding assay.
This approach enables you to identify the most promising ligands early on saving time and resources while ensuring high-quality data for downstream optimization and minimizing the need for animal studies.
Gained information
- Precise hit selection based on kinetic behavior
- Comparative ranking of binding affinities
- Evaluation of ligand modifications (e.g., mutations, tags, conjugates) on binding kinetics
- Evaluation of binding mechanism
Key publications
These publications illustrate the application well. Click here for more examples
Affinity maturation and optimization of CD44v6-targeting antibodies for molecular radiotherapy.
Mortensen ACL, Hofström C, Persson H, Dahllund L, Frejd FY, Nestor M.
Nucl Med Biol. 2025. 144-145:109012.
Summary
PubMed
Design, Synthesis, and Biological Evaluation of Small-Molecule-Based Radioligands with Improved Pharmacokinetic Properties for Imaging of Programmed Death Ligand 1.
Krutzek F, Donat CK, Ullrich M, Stadlbauer S.
J Med Chem. 2023. 66(23):15894-15915.
Summary
PubMed
Selection of an optimal cysteine-containing peptide-based chelator for labeling of affibody molecules with 188Re.
Altai M, Honarvar H, Wållberg H, Strand J, Varasteh Z, Rosestedt M, Orlova A, Dunås F, Sandström M, Löfblom J, Tolmachev V, Ståhl S.
Eur J Med Chem. 2014. 87:519-528.
Summary
PubMed
Examples of insights
