Physiological processes
Study ligand – cell interactions in a physiological environment
Investigate how the strength and kinetics of your interaction is affected by temperature. Reduce the temperature to turn off cellular processes such as internalization, or increase it to 37 °C to approach an in vivo setting. Understand your system better with the use of labels, such as pH sensitive dyes or radionuclides that can be excreted.
Gained information
- Temperature effects on the affinity
- Temperature effects on the binding kinetics
- Signs of internalization rate
- Nuclide excretion rate as the result of internalization, degradation, or cell death.
Key publications
These publications illustrate the application well. Click here for more examples.
Resolving the EGF-EGFR interaction characteristics through a multiple-temperature, multiple-inhibitor, real-time interaction analysis approach.
Björkelund H, Gedda L, Malmqvist M, Andersson K.
Mol Clin Onc. 2013. 1: 343-352
Summary
PubMed
Helicobacter pylori adapts to chronic infection and gastric disease via pH-Responsive BabA-mediated adherence.
Bugaytsova JA et al.
Cell Host Microbe. 2017. 21(3):376-389.
Summary
PubMed
Effect of clofarabine on apoptosis and DNA synthesis in human epithelial colon cancer cells.
Wang X, Albertioni F.
Nucleosides Nucleotides Nucleic Acids. 2010. 29(4-6):414-418.
Summary
PubMed
A real‐time cell‐binding assay reveals dynamic features of STxB–Gb3 cointernalization and STxB‐mediated cargo delivery into cancer cells.
Crispim Encarnação J, Napolitano V, Opassi G, Danielson HU, Dubin G, Popowicz GM, Munier‐Lehmann H, Buijs J, Andersson K, Björkelund H.
FEBS Letters. 2020. 594(15): 2406-2420.
Summary
PubMed
Examples of insights
