Receptor clustering and dimerization
Understand receptor clustering and dimerization
A ligand-target interaction often changes in affinity and kinetics when the receptor shifts from a monomeric to a dimeric state, or when it forms clusters. This can be exploited to better understand your biological system. Investigate how drugs induce or prohibit dimerization or clustering by following the ligand binding, or label some ligands with quenchers* and study changes in proximity over time.
*only in LigandTracer Green
Gained information
- Proximity of ligands caused by target clustering and dimerization
- Effects of drugs inducing or preventing target clustering and dimerization
- The impact of receptor populations on target clustering and dimerization
- Avidity effects
Resources
Key publications
These publications illustrate the application well. Click here for more examples.
A novel real-time proximity assay for characterizing multiple receptor interactions on living cells.
Bondza S, Björkelund H, Nestor M, Andersson K, Buijs J.
Anal Chem. 2017. 89(24):13212-13218
Summary
PubMed
Gefitinib induces epidermal growth factor receptor dimers which alters the interaction characteristics with 125I-EGF.
Björkelund H, Gedda L, Barta P, Malmqvist M, Andersson K.
PLoS ONE. 2011. 6(9): e24739.
Summary
PubMed
Examples of insights
