Virus, bacteria and cells
Monitor the avidity of cell-cell interactions and uptake of bacteria or virus
The concept of ligand is not limited to molecules. In fact, as long as the compound is labeled and in solution it can even be a living or semi-living thing, such as a cell, bacteria or virus. Monitor multivalent binding and investigate the impact of e.g. pH, peptide presentation or presence of additional compounds in the medium.
Key applications
- Study uptake and retention of viral particles or bacteria
- Validate bispecific molecules (cell – bispecific binder – cell)
- Compare engagement of immune cells with target cells (e.g. CAR T-cells)
Key publications
These publications illustrate the application well. Click here for more examples.
Adenovirus with hexon Tat-PTD modification exhibits increased therapeutic effect in experimental neuroblastoma and neuroendocrine tumors.
Yu D, Jin C, Leja J, Majdalani N, Nilsson B, Eriksson F, Essand M.
J Virol. 2011. 85(24):13114-13123.
Summary
PubMed
T4SS-dependent TLR5 activation by Helicobacter pylori infection
Pachathundikandi SK, Tegtmeyer N, Arnold IC, Lind J, Neddermann M, Falkeis-Veits C, Chattopadhyay S, Bronstrup M, Tegge W, Hong M, Sticht H, Vieth M, Muller A, Backert S.
Nat Commun. 2019. 10: 5717.
Summary
PubMed
Potent Fc Receptor Signaling by IgA Leads to Superior Killing of Cancer Cells by Neutrophils Compared to IgG.
Brandsma AM, Bondza S, Evers M, Koutstaal R, Nederend M, Jansen M, Rösner T, Valerius T, Leusen JHW, Broeke T.
Front Imm. 2019. 10:704.
Summary
PubMed
Examples of ligands
