Protein
Protein – protein interactions measured with LigandTracer
Working with purified proteins is suitable when the target protein is not expressed on a cell surface. The cell-free environment also enables a highly controlled setting, ideal for quality control measurements. The target proteins may be attached through adhesion, immobilized on a chip or on magnetic beads.
Key applications
- Estimate kinetics and affinity
- Concentration estimation
- Investigate specificity and competitive properties
- Evaluate how conjugations affect the ligand
- Quality control of batches, over time, etc.
Key publications
These publications illustrate the application well. Click here for more examples.
SPECT imaging of distribution and retention of a brain-penetrating bispecific amyloid-β antibody in a mouse model of Alzheimer’s disease.
Gustavsson T, Syvänen S, O’Callaghan P, Sehlin D.
Transl Neurodegener. 2020. 9:37.
Summary
PubMed
Automated functional characterization of radiolabeled antibodies: a time-resolved approach.
Wang E, Björkelund H, Mihaylova D, Hagemann UB, Karlsson J, Malmqvist M, Buijs J, Abrahmsén L, Andersson K.
Nucl Med Commun. 2014. 35(7):767-776.
Summary
PubMed
Examples of targets
