On Adherent cells
Study how your binder interacts with surface receptors expressed on live cells
The cellular environment offers a step towards in vivo assays with the presence of the dynamic cell membrane, secondary binding partners and internalization/degradation processes that may be turned on and off with temperature changes. Seeding the cells is straightforward – use regular Petri dishes, or LigandTracer cell culture MultiDish 2×2 for higher throughput and comparative studies* – and let the inherent properties of the cells do the rest.
Key applications
- Estimate kinetics and affinity
- Investigate specificity and competitive properties
- Study receptor clustering and dimerization
- Characterize drug conjugates
- Analyze the impact of physiological processes
Resources
Key publications
These publications illustrate the application well. Click here for more examples.
Feasibility of Imaging EpCAM Expression in Ovarian Cancer Using Radiolabeled DARPin Ec1.
Vorobyeva A, Konovalova E, Xu T, Schulga A, Altai M, Garousi J, Rinne SS, Orlova A, Tolmachev V, Deyev S.
Int J Mol Sci. 2020. 21(9): 3310.
Summary
PubMed
New insights in Type I and II CD20 antibody mechanisms-of-action with a panel of novel CD20 antibodies.
Meyer S, Evers M, Jansen JHM, Buijs J, Broek B, Reitsma SE, Moerer P, Amini M, Kretschmer A, Ten Broeke T, den Hartog MT, Rijke M, Klein C, Valerius T, Boross P, Leusen JHW.
Front. Immunol. 2017. 8(455).
Summary
PubMed
Examples of targets
